These candidates affecting heart mutant phenotype are to be identified 這些影響心臟突變表型的候選基因還需要進(jìn)一步鑒定。
The chimeric gene was transformed into rice calli by particle bombardment . many resistance calli were obtained , and gus activity was detected . in order to confirm the causality of the mutant phenotype and osdd2 gene , we took an approach of functional knockout ofosdd2 gene , with the method rnai ( rna interference ) 為了進(jìn)一步驗證本基因的功能,利用獲得的包含wrky保守域的cdna片斷構(gòu)建了可能產(chǎn)生rna干涉( rnainterference , rnai )的載體,利用農(nóng)桿菌轉(zhuǎn)化法轉(zhuǎn)化水稻,目前已經(jīng)獲得一批轉(zhuǎn)基因苗。
Using conjunction mobilization , we screened the mutant of eaggec 17 - 2 with the mutant phenotype by one step . compared mutant and eaggec 17 - 2 , the experiments such as growth under iron - deficient conditions , virulence testing to the mouse , and ybt biosynthesis and regulation showed that ybtp , ybtq and ybtx genes have no significant effects on iron uptake , mouse virulen Eaggec17毛攜帶的hpi毒力島的功能研究eaggec172的ybtp 、 ybtqyybtx突變株沒有表現(xiàn)出鐵缺陷培養(yǎng)條件下的生長抑制表型,對ybt的合成無明顯影響;對野生株與ybtp突變株的鼠毒力對比實驗表明, lds 。